| Download | - View final version: Cellular delivery of plasmid DNA into wheat microspores using rosette nanotubes (PDF, 3.0 MiB)
- View supplementary information: Cellular delivery of plasmid DNA into wheat microspores using rosette nanotubes (PDF, 669 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1021/acsomega.0c02830 |
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| Author | Search for: Cho, Jae-Young1; Search for: Bhowmik, Pankaj2; Search for: Polowick, Patricia L.2; Search for: Dodard, Sabine G.2; Search for: El-Bakkari, Mounir1; Search for: Nowak, Goska2; Search for: Fenniri, Hicham1ORCID identifier: https://orcid.org/0000-0001-7629-7080; Search for: Hemraz, Usha D.2ORCID identifier: https://orcid.org/0000-0001-5151-9484 |
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| Affiliation | - National Research Council of Canada. Nanotechnology
- National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | genetics; monomers; organic polymers; fluorescence; gene delivery |
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| Abstract | Plant genetic engineering offers promising solutions to the increasing demand for efficient, sustainable, and high-yielding crop production as well as changing environmental conditions. The main challenge for gene delivery in plants is the presence of a cell wall that limits the transportation of genes within the cells. Microspores are plant cells that are, under the right conditions, capable of generating embryos, leading to the formation of haploid plants. Here, we designed cationic and fluorescent rosette nanotubes (RNTs) that penetrate the cell walls of viable wheat microspores under mild conditions and in the absence of an external force. These nanomaterials can capture plasmid DNA to form RNT–DNA complexes and transport their DNA cargo into live microspores. The nanomaterials and the complexes formed were nontoxic to the microspores. |
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| Publication date | 2020-09-16 |
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| Publisher | American Chemical Society |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-ACRD PDB059 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | e168e842-4aba-4349-8a5a-a8b6df57eff6 |
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| Record created | 2020-10-23 |
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| Record modified | 2020-10-23 |
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