Simultaneous quantitation from infrared spectra of glucose concentrations, lactate concentrations, and lecithin/sphingomyelin ratios in amniotic fluid

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1016/S0002-9378(99)70275-9
Author	Search for: Liu, Kan-Zhi¹; Search for: Mantsch, Henry H.¹
Affiliation	National Research Council Canada. NRC Institute for Biodiagnostics
Format	Text, Article
Subject	Amniotic fluid; fetal distress; glucose; infrared spectroscopy; lactate; preterm infection
Abstract	Objective: The purpose of this study was to assess the feasibility of using infrared spectroscopy to simultaneously predict preterm infection, fetal distress, and fetal lung maturity. Study Design: A total of 189 infrared spectra were acquired from amniotic fluid obtained by amniocentesis. The concentrations of glucose and lactate and the lecithin/sphingomyelin ratios were determined separately by accepted clinical chemistry methods for each sample. Infrared spectra were recorded with a commercial spectrometer and 35 μL amniotic fluid was used for each spectrum. Calibration models (partial least squares) were derived from the correlation between 102 infrared spectra and clinical standard analyses; the model was then validated with the remaining 87 spectra. Results: By means of the multivariate technique of partial least squares regression, calibration models for glucose and lactate were developed that had excellent correlation coefficients (r = 0.97 for glucose and r = 0.91 for lactate); the SEs of calibration were 0.04 mmol/L for glucose and 0.09 mmol/L for lactate. The validation sets for the quantitation of glucose and lactate predicted by the calibration models also yielded good outcomes (r = 0.95 for glucose and r = 0.71 for lactate, with SEs of prediction of 0.06 mmol/L and 0.18 mmol/L, respectively). Conclusion: Infrared spectroscopy has the potential to become the clinical method of choice for simultaneously predicting preterm infection, fetal distress, and fetal lung maturity.
Publication date	1999-03
In	American Journal of Obstetrics and Gynecology 180, no. 3, part 1: 696–702.
Peer reviewed	Yes
NRC number	919 NRCC 34806
NPARC number	9148134
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Record identifier	e1e30bd9-3b26-41a0-86cb-0ef7dbf854b6
Record created	2009-06-25
Record modified	2020-03-20

Date modified:: 2026-01-21