Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/o05-063
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Name affiliation
  1. National Research Council of Canada. Aquatic and Crop Resource Development
FormatText, Article
Journal titleBiochemistry and Cell Biology
ISSN0829-8211
1208-6002
Volume83
Issue5
Pages637–643
Subjectblueberry flavonoids; MMP activity; prostate cancer cells
Abstract
Regulation of the matrix metalloproteinases (MMPs), the major mediators of extracellular matrix (ECM) degradation, is crucial to regulate ECM proteolysis, which is important in metastasis. This study examined the effects of 3 flavonoid-enriched fractions (a crude fraction, an anthocyanin-enriched fraction, and a proanthocyanidin-enriched fraction), which were prepared from lowbush blueberries (Vaccinium angustifolium), on MMP activity in DU145 human prostate cancer cells in vitro. Using gelatin gel electrophoresis, MMP activity was evaluated from cells after 24-hr exposure to blueberry fractions. All fractions elicited an ability to decrease the activity of MMP-2 and MMP-9. Of the fractions tested, the proanthocyanidin-enriched fraction was found to be the most effective at inhibiting MMP activity in these cells. No induction of either necrotic or apoptotic cell death was noted in these cells in response to treatment with the blueberry fractions. These findings indicate that flavonoids from blueberry possess the ability to effectively decrease MMP activity, which may decrease overall ECM degradation. This ability may be important in controlling tumor metastasis formation.
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Copyright statement
  • © 2005 NRC Canada
LanguageEnglish
Peer reviewedYes
NRC numberNRC-IMB-1711
NPARC number3538300
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