Studies on the hyperplasia ('regeneration') of the rat liver following partial hepatectomy. Changes in lipid peroxidation and general biochemical aspects

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1042/bj2650051
Author	Search for: Slater, T.F.; Search for: Cheeseman, K.H.; Search for: Benedetto, C.; Search for: Collins, M.; Search for: Emery, S.; Search for: Maddix, S.P.; Search for: Nodes, J.T.; Search for: Proudfoot, K.; Search for: Burton, G.W.; Search for: Ingold, K. U.¹
Name affiliation	National Research Council of Canada
Format	Text, Article
Subject	lipid peroxidase; thymidine kinase; animal cell; animal experiment; liver microsome; liver regeneration; liver resection; antioxidants; carbon tetrachloride; cytochrome P-450 enzyme system; DNA; fatty acids; hepatectomy; hyperplasia; lipid peroxidation; lipids; liver; liver Regeneration; male; microsomes, liver; NADPH-ferrihemoprotein reductase; oxygen consumption; proteins; rats; rats, inbred strains; support, Non-U.S. Gov't; thymidine Kinase; vitamin E; animalia
Abstract	Using the experimental model of partial hepatectomy in the rat, we have examined the relationship between cell division and lipid peroxidation activity. In rats entrained to a regime of 12 h light/12 h dark and with a fixed 8 h feeding period in the dark phase, partial hepatectomy is followed by a rapid regeneration of liver mass with cycles of synchronized cell division at 24 h intervals. The latter phenomenon is indicated in this study by pulses of thymidine kinase activity having maxima at 24 h, 48 h and 72 h after partial hepatectomy. Microsomes prepared from regenerating livers show changes in lipid peroxidation activity (induced by NADPH/ADP/iron or by ascorbate/iron), which is significantly decreased relative to that in microsomes from sham-operated controls, again at 24 h, 48 h and 72 h after the operation. This phenomenon has been investigated with regard to possible underlying changes in the content of microsomal fatty acids, the microsomal enzymes NADPH:cytochrome c reductase and cytochrome P-450, and the physiological microsomal antioxidant α-tocopherol. The cycles of decreased lipid peroxidation activity are apparently due, at least in part, to changes in microsomal α-tocopherol content that are closely associated in time with thymidine kinase activity.
Publication date	1990
In	Biochemical Journal 265, no. 1 (1990): 51–59.
Language	English
Peer reviewed	Yes
NPARC number	21276749
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Record identifier	e371fa40-c25c-4724-bf81-23f6a10d7b8e
Record created	2015-10-13
Record modified	2020-03-17

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