Studies on the hyperplasia ('regeneration') of the rat liver following partial hepatectomy. Changes in lipid peroxidation and general biochemical aspects

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1042/bj2650051
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Name affiliation
  1. National Research Council Canada
FormatText
TypeArticle
Journal titleBiochemical Journal
ISSN0264-6021
Volume265
Issue1
Pages51–59; # of pages: 9
Subjectlipid peroxidase; thymidine kinase; animal cell; animal experiment; liver microsome; liver regeneration; liver resection; rat; Antioxidants; Carbon Tetrachloride; Cytochrome P-450 Enzyme System; DNA; Fatty Acids; Hepatectomy; Hyperplasia; Lipid Peroxidation; Lipids; Liver; Liver Regeneration; Male; Microsomes, Liver; NADPH-Ferrihemoprotein Reductase; Oxygen Consumption; Proteins; Rats; Rats, Inbred Strains; Support, Non-U.S. Gov't; Thymidine Kinase; Vitamin E; Animalia
Abstract
Using the experimental model of partial hepatectomy in the rat, we have examined the relationship between cell division and lipid peroxidation activity. In rats entrained to a regime of 12 h light/12 h dark and with a fixed 8 h feeding period in the dark phase, partial hepatectomy is followed by a rapid regeneration of liver mass with cycles of synchronized cell division at 24 h intervals. The latter phenomenon is indicated in this study by pulses of thymidine kinase activity having maxima at 24 h, 48 h and 72 h after partial hepatectomy. Microsomes prepared from regenerating livers show changes in lipid peroxidation activity (induced by NADPH/ADP/iron or by ascorbate/iron), which is significantly decreased relative to that in microsomes from sham-operated controls, again at 24 h, 48 h and 72 h after the operation. This phenomenon has been investigated with regard to possible underlying changes in the content of microsomal fatty acids, the microsomal enzymes NADPH:cytochrome c reductase and cytochrome P-450, and the physiological microsomal antioxidant α-tocopherol. The cycles of decreased lipid peroxidation activity are apparently due, at least in part, to changes in microsomal α-tocopherol content that are closely associated in time with thymidine kinase activity.
Publication date
LanguageEnglish
Peer reviewedYes
NPARC number21276749
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