| DOI | Resolve DOI: https://doi.org/10.1016/j.bbrc.2013.05.068 |
|---|
| Author | Search for: He, Yonghan1; Search for: Li, Ying; Search for: Zhang, Shuocheng1; Search for: Perry, Ben1; Search for: Zhao, Tiantian1; Search for: Wang, Yanwen1; Search for: Sun, Changhao |
|---|
| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
|
|---|
| Format | Text, Article |
|---|
| Subject | Radicicol; Heat shock protein; Differentiation; Adipocytes |
|---|
| Abstract | Heat shock protein 90 (Hsp90) is involved in various cellular processes, such as cell proliferation, differentiation and apoptosis. As adipocyte differentiation plays a critical role in obesity development, the present study investigated the effect of an Hsp90 inhibitor radicicol on the differentiation of 3T3-L1 preadipocytes and potential mechanisms. The cells were treated with different concentrations of radicicol during the first 8 days of cell differentiation. Adipogenesis, the expression of adipogenic transcriptional factors, differentiation makers and cell cycle were determined. It was found that radicicol dose-dependently decreased intracellular fat accumulation through down-regulating the expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT element binding protein α (C/EBPα), fatty acid synthase (FAS) and fatty acid-binding protein 4 (FABP4). Flow cytometry analysis revealed that radicicol blocked cell cycle at G1-S phase. Radicicol redcued the phosphorylation of Akt while showing no effect on β-catenin expression. Radicicol decreased the phosphorylation of phosphoinositide-dependent kinase 1 (PDK1). The results suggest that radicicol inhibited 3T3-L1 preadipocyte differentiation through affecting the PDK1/Akt pathway and subsequent inhibition of mitotic clonal expansion and the expression/activity of adipogenic transcriptional factors and their downstream adipogenic proteins. |
|---|
| Publication date | 2013-05-28 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NRC number | NRCC 55483 |
|---|
| NPARC number | 21268615 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | e63e9088-3f5e-46ba-91ad-50c882abe7fe |
|---|
| Record created | 2013-10-28 |
|---|
| Record modified | 2020-04-22 |
|---|
