| Alternative title | Analysis of sequence tags in Brassica cotyledons damaged by crucifer flea beetle feeding |
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| DOI | Resolve DOI: https://doi.org/10.1139/g11-083 |
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| Author | Search for: Gruber, Margaret; Search for: Wu, Limin; Search for: Links, Matthew; Search for: Gjetvaj, Branimir; Search for: Durkin, Johnathan; Search for: Lewis, Christopher; Search for: Sharpe, Andrew1; Search for: Lydiate, Derek; Search for: Hegedus, Dwayne |
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| Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
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| Format | Text, Article |
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| Subject | Brassica napus; cotyledon EST representation; flea beetle Phyllotreta; host plant molecular response; catalogue d'EST chez le cotylédon; altise des crucifères Phyllotetra; réponses moléculaires de l'hôte |
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| Abstract | The molecular basis of canola (Brassica napus L.) susceptibility to the crucifer flea beetle (FB, Phyllotreta cruciferae Goeze) was investigated by comparing transcript representation in FB-damaged and undamaged cotyledons. The B. napus cotyledon transcriptome increased and diversified substantially after FB feeding damage. Twenty-two genes encoding proteins with unknown function, six encoding proteins involved in signaling, and a gene encoding a B-box zinc finger transcription factor were moderately or strongly changed in representation with FB feeding damage. Zinc finger and calciumdependent genes formed the largest portion of transcription factors and signaling factors with changes in representation. Six genes with unknown function, one transcription factor, and one signaling gene specific to the FB-damaged library were co-represented in a FB-damaged leaf library. Out of 188 transcription factor and signaling gene families screened for “early” expression changes, 16 showed changes in expression within 8 h. Four of these early factors were zinc finger genes with representation only in the FB-damaged cotyledon. These genes are now available to test their potential at initiating or specifying cotyledon responses to crucifer FB feeding. |
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| Publication date | 2012-01-25 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 54632 |
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| NPARC number | 21268458 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | e795a846-b518-4116-af47-b3f8b8e18ee6 |
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| Record created | 2013-07-24 |
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| Record modified | 2020-04-21 |
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