Metabolic and hormonal processes associated with the induction of secondary dormancy in Brassica napus seeds

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DOI

Resolve DOI: https://doi.org/10.1139/B09-022

Author

Search for: Fei, Houman; Search for: Ferhatoglu, Yurdagul; Search for: Tsang, Edward¹; Search for: Huang, Daiqing¹; Search for: Cutler, Adrian J.¹

Affiliation

National Research Council of Canada. NRC Plant Biotechnology Institute

Format

Text, Article

Subject

canola; microarray; metabolite profiling; abscisic acid; polyethylene glycol; lipids; phenylpropanoid; dormancy

Abstract

Polyethylene glycol treatment induces secondary seed dormancy in Brassica napus L. cultivar ‘AC Excel’ (ACE), but not in ‘DH12075’ (DH). Gene expression, metabolite profiles, and hormone profiles were obtained from seeds of both cultivars following polyethylene glycol 8000 treatment. ACE seeds were more transcriptionally active: 28 genes were up-regulated in both cultivars and 10 and 158 genes were specifically up-regulated in DH and ACE, respectively. Nontargeted metabolite analyses combined with gene expression analyses showed significant differences in lipid, sugar, and phenylpropanoid metabolism between the cultivars. Abscisic acid (ABA) levels were higher and many ABA-inducible genes were expressed more in ACE. An association of ABA with secondary dormancy was supported by the observation that secondary dormancy was induced by polyethylene glycol 8000 in Arabidopsis wild-type seeds, but was reduced in ABA-deficient and ABA-insensitive mutants. Therefore, secondary dormancy appears to be realized through an active ABA-related mechanism that may involve changes in primary and secondary metabolism.

Publication date

2009-06

Publisher

National Research Council of Canada. NRC Research Press

Language

English

Peer reviewed

Yes

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Record identifier

e8423c85-c955-456a-a4b7-e91c87e73493

Record created

2019-03-07

Record modified

2023-01-25

Date modified:: 2024-07-19