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On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1002/(SICI)1097-0290(19990405)63:1<122::AID-BIT13>3.0.CO;2-I
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Affiliation
  1. National Research Council of Canada. NRC Biotechnology Research Institute
FormatText, Article
Subjectcapacitance; permittivity; on-line monitoring; baculovirus; insect cells
Abstract
The use of on-line relative permittivity (ϵ′) measurements for monitoring cultures of Sf-9 cells was evaluated in a batch culture and a batch infected with a baculovirus expressing β-galactosidase. It was found that viable cell density and volume essentially accounted for all the variation in ϵ′ in both non-infected and synchronously infected cultures, indicating that the ϵ′ of a cell suspension was sensitive only to changes in the viable cell population. Additionally the parameter provided clearly defined signposts of the progress of the infection.
Publication date
PublisherWiley
In
  • Biotechnology and Bioengineering 63, no. 1 (April 1999): 122–126.
Noteyes
LanguageEnglish
NRC number37675
NPARC number3538808
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Record identifiereb4b2a13-4eee-4bca-94ac-cc366ff27e74
Record created2009-03-01
Record modified2022-03-03
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