National Research Council of Canada. NRC Biotechnology Research Institute
CRWAD, December 2007, Chicago, IL, USA
The primary aim of this study was to create a diagnostic microarray for the identification of Brucella sp. of clinical importance in both the medical and veterinary field. The microarray should be able to identify other organisms that may cause abortions in animals or that may elicit an immunological response similar to that of Brucella sp.
Oligonucleotide probes were designed specific to the most common species found to cause zoonotic pathologies; the sequences were designed from the following genes; 16S rRNA, 16S-23S rRNA intergenic transcribed spacer region (ITS), β-subunit of the DNA-dependent RNA polymerase (rpoβ), heat shock protein (hsp), gyrase beta (gyrβ) and from other genes usually used as PCR targets. This microarray also contains virulence factor genes specific to the Brucella sp., Preliminary results confirmed the microarray as an effective one-step method for the identification of Brucella sp. from culture even if, the validation is still in progress.