| DOI | Resolve DOI: https://doi.org/10.1021/bi802100u |
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| Author | Search for: Houliston, R. Scott1; Search for: Jacobs, Bart C.; Search for: Tio-Gillen, Anne P.; Search for: Verschuuren, Jan J.; Search for: Khieu, Nam H.1; Search for: Gilbert, Michel1; Search for: Jarrell, Harold C.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | acid; antibodies, anti-idiotypic; antibody; autoantibodies; binding; biophysical processes; blood; Canada; diagnosis; disaccharide; enzyme-linked immunosorbent assay; epitope; epitope mapping; epitopes; form; fractionation; ganglioside; high-resolution; IGM; immunoglobulins; immunology; interaction; ligand; ligands; methods; models, molecular; molecular; nuclear magnetic resonance, biomolecular; polyneuropathies; region; sensitivity and specificity; serum; sialic; sialic-acid; signal; specificity; target; unit |
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| Abstract | High-resolution binding profiles were elucidated for anti-GM1 IgM autoantibodies from two patients with a progressive form of paraproteinemic polyneuropathy. Antibody-ligand interaction was characterized by generating STD-NMR signals in target ganglio-oligosaccharides added directly to patient sera, without the requirement of antibody fractionation. Both immunoglobulins were found to have similar binding modalities, with interaction confined to two distinct spatially separated regions of GM1: the terminal betaGal(1-3)betaGalNAc disaccharide unit and the sialic acid residue. We describe a unique and powerful biophysical technique applied to define the molecular interaction between autoimmune disease-causing antibodies and their ganglioside targets |
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| Publication date | 2009-01-20 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | HOULISTON2009 |
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| NPARC number | 9359763 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | edc83d6f-8b9a-41f5-af67-d59b35b94579 |
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| Record created | 2009-07-10 |
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| Record modified | 2020-04-16 |
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