Atlantic halibut (Hippoglossus hippoglossus) and Senegal sole (Solea senegalensis) are two flatfishes yielding high value market products with good potential for aquaculture in eastern North America and Mediterranean Europe, respectively. Production–related problems in these two evolutionary–related species may be addressed with improved knowledge of important basic biological processes such as reproduction, development, nutrition, genetics and immunity. The use of genomic approaches to thoroughly characterize these processes will translate into knowledge that can be used to overcome the production obstacles and create (for PLEUROGENE is a new initiative funded through Genome Canada-Genome Spain for three years (2004-2007). There are two main goals: (i) the construction of genetic linkage maps Atlantic halibut and Senegal sole for use in the selection of improved broodstock based on molecular markers, and (ii) design, construction a flatfish microarray studies gene expression these two species. High-throughput genome- proteome-based technologies will be used identification, characterization mapping genes important reproduction, larval development, immunity nutrition. All the genetic and molecular information obtained in this project will be integrated into an interactive bioinformatic platform specifically developed for the project. The knowledge generated by the PLEUROGENE project will ultimately lead to the establishment of new technologies for the control of reproduction and optimization of larval health and nutrition in the Senegal sole, Atlantic halibut, and other related flatfish species under intensive culture conditions.