| DOI | Resolve DOI: https://doi.org/10.1016/j.micres.2010.12.002 |
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| Author | Search for: Feng, J.; Search for: Bhadauria, V.; Search for: Liu, G.; Search for: Selvaraj, G.1; Search for: Hughes, G.R.; Search for: Wei, Y. |
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| Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
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| Format | Text, Article |
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| Subject | Basal expression; Cis-acting element; Cis-regulatory elements; Deletion mutants; Flanking sequence; Fusarium graminearum; Fusarium head blights; Gene induction; Gene regulations; GFP expression; Green fluorescent protein; In-silico; Long-chain fatty acids; Open reading frame; Promoter activities; Promoter region; Promoter sequences; Regulatory elements; Encoding (symbols); Fluorescence; Gene encoding; Gene expression; Glucose; Glycerol; Lipases; Fatty acids; green fluorescent protein; triacylglycerol lipase; alcohol; biodegradation; catalysis; enzyme activity; ester; fatty acid; fluorescence; fungus; gene expression; mutation; pathogen; binding site; enzymology; Fusarium; gene deletion; gene fusion; genetics; metabolism; regulatory sequence; reporter gene; wheat; Artificial Gene Fusion; Binding Sites; Fusarium; Genes, Reporter; Green Fluorescent Proteins; Lipase; Regulatory Sequences, Nucleic Acid; Sequence Deletion; Triticum; Fusarium; Gibberella zeae; Triticum aestivum |
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| Abstract | Triglyceride lipases catalyze the reversible degradation of glycerol esters with long-chain fatty acids into fatty acids and glycerol. In silico analysis of 5'-end flanking sequence of the gene LIP1 encoding a triglyceride lipase from the wheat head blight pathogen Fusarium graminearum revealed the presence of several cis-regulatory elements. To delineate the function of these regulatory elements, we constructed a series of deletion mutants in the LIP1 promoter region fused to the open reading frame of a green fluorescent protein (GFP) and assayed the promoter activity. Analysis of GFP expression levels in mutants indicated that a 563-bp promoter sequence was sufficient to drive the expression of LIP1 and regulatory elements responsible for the gene induction were located within the 563-372. bp region. To further investigate the regulatory elements, putative cis-acting elements spanned within the 563-372. bp region were mutated using a targeted mutagenesis approach. A CCAAT box, a CreA binding site, and a fatty acid responsive element (FARE) were identified and confirmed to be required for the basal expression of LIP1, glucose suppression and fatty acid induction, respectively. © 2011 Elsevier GmbH. |
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| Publication date | 2011 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21271088 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | efb3b093-9a19-42a5-8aeb-50d33c9081c8 |
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| Record created | 2014-03-24 |
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| Record modified | 2020-04-21 |
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