| Abstract | Cell-wall lipopolysaccharide isolated from Pasteurella haemolytica serotype T3 using the phenol–water extraction procedure was shown to be an S type lipopolysaccharide by sodium dodecyl sulphate–polyacrylamide gel electrophoresis. Hydrolysis with mild acid afforded a lipid-free, antigenic O-chain polysaccharide. On the basis of one- and two-dimensional 1H and 13C nuclear magnetic resonance studies, in conjunction with microanalytical chemical methods, the O-polysaccharide was determined to be a linear polymer of a disaccharide repeating unit having the structure (Formula: see text). |
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