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Nutrient requirements of suspension cultures of soybean root cells

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/0014-4827(68)90403-5
AuthorSearch for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
Abstract
The nutrient requirements of suspension cultures from soybean root have been investigated, and a simple medium consisting of mineral salts, sucrose, vitamins and 2,4-dichlorophenoxyacetic acid (2,4₋d) has been designed. The cells required thiamine, 2,4₋d and ammonium in addition to the usual mineral salts and sucrose. Optimum concentrations of nitrate and ammonium were 25 and 2 mM respectively. The highest yield of cells was achieved at an initial pH of 4.5–5.5. During the growth cycle the pH gradually increased to 6.0–6.2.
Publication date
PublisherElsevier
In
  • Experimental Cell Research 50, no. 1 (April 1968): 151–158.
LanguageEnglish
Peer reviewedYes
Identifier0014482768904035
NRC numberNRCC 9901
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Record identifierf294588d-1d90-4a35-9881-52cd31bf0140
Record created2023-07-27
Record modified2023-07-27
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