| DOI | Resolve DOI: https://doi.org/10.1073/pnas.1222317110 |
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| Author | Search for: Willis, L.M.; Search for: Stupak, J.1; Search for: Richards, M.R.; Search for: Lowary, T.L.; Search for: Li, J.1; Search for: Whitfield, C. |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | ABC transporter; glycerophospholipid; glycolipid; glycosaminoglycan polysulfate; polysaccharide; polysialic acid; amino acid sequence; bacterial capsule; biosynthesis; Escherichia coli; hydrophobicity; lipid analysis; molecular weight; Neisseria meningitidis; proton nuclear magnetic resonance; capsule assembly; polysaccharide export; Adenosine Triphosphate; ATP-Binding Cassette Transporters; Bacterial Capsules; Biological Transport; Escherichia coli; Glycolipids; Gram-Negative Bacteria; Magnetic Resonance Spectroscopy; Mass Spectrometry; Methylation; Mutation; Neisseria meningitidis; Polysaccharides; Virulence Factors |
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| Abstract | Bacterial capsules are surface layers made of long-chain polysaccharides. They are anchored to the outer membrane of many Gramnegative bacteria, including pathogens such as Escherichia coli, Neisseriameningitidis, Haemophilus influenzae, andPasteurella multocida. Capsules protect pathogens from host defenses including complement- mediated killing and phagocytosis and therefore represent a major virulence factor. Capsular polysaccharides are synthesized by enzymes located in the inner (cytoplasmic) membrane and are then translocated to the cell surface.Whereas the enzymes that synthesize the polysaccharides have been studied in detail, the structure and biosynthesis of the anchoring elements have not been definitively resolved. Here we determine the structure of the glycolipid attached to the reducing terminus of the polysialic acid capsular polysaccharides from E. coli K1 and N. meningitidis group B and the heparosan-like capsular polysaccharide from E. coli K5. All possess the same unique glycolipid terminus consisting of a lyso-phosphatidylglycerol moiety with a β-linked poly-(3-deoxy-D-manno-oct-2-ulosonic acid) (poly-Kdo) linker attached to the reducing terminus of the capsular polysaccharide. |
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| Publication date | 2013 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270615 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | f820ac5a-63c5-40ff-8c30-857e9f9cbb2e |
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| Record created | 2014-02-17 |
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| Record modified | 2022-11-18 |
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