High-performance liquid chromatography of domoic acid, a marine neurotoxin, with application to shellfish and plankton

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1080/03067318908026867
Author	Search for: Quilliam, Michael A.¹ ; Search for: Sim, P. Greig¹ ; Search for: Mcculloch, Archibald W.¹ ; Search for: Mcinnes, A. Gavin¹
Name affiliation	National Research Council Canada
Format	Text
Type	Article
Journal title	International Journal of Environmental Analytical Chemistry
ISSN	0306-7319 1029-0397
Volume	36
Issue	3
Pages	139–154
Subject	domoic acid; neurotoxin; shellfish; high-performance liquid chromatography; ultraviolet diode array detector
Abstract	A recent outbreak of poisoning resulting from the consumption of cultured blue mussels (Mytilus edulis L.) from a localized area in Eastern Canada has been attributed to the presence of domoic acid (1), a relatively rare neurotoxic amino acid, previously found only in some algae of the family Rhodomelaceae. Studies on aqueous extracts of shellfish tissue indicated that the toxin and several of its isomers could be separated (and isolated in sufficient amounts for subsequent structural identification) by reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) diode array detection (DAD). Aqueous acetonitrile containing 0.1% v/v trifluoroacetic acid was used as mobile phase. As the retention time and characteristic UV absorption spectrum of 1 (λmax = 242 nm) permit unequivocal identification, the HPLC-DAD procedure was refined with a microbore column to provide a rapid (5 min), sensitive (0.3 ng detection limit) and reproducible assay method for the determination of 1 in shellfish tissue. Extraction was accomplished by boiling homogenized shellfish tissue for 5 min with distilled water. Extracts were taken through an octadecylsilica solid phase extraction clean-up prior to HPLC. This method has been applied to a variety of shellfish and phytoplankton samples.
Publication date	1989-07
Publisher	Taylor and Francis
Terms of use	Permission is granted to temporarily download one copy of the materials for personal, noncommercial transitory viewing only. NRC Publications Archive - Copyright and terms of use
Language	English
Peer reviewed	Yes
NPARC number	23000945
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Record identifier	fb7db56b-eab3-4e63-abf8-4c7c6fc93155
Record created	2016-11-21
Record modified	2016-11-21

Date modified:: 2019-03-22

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