| DOI | Resolve DOI: https://doi.org/10.1094/Phyto-65-826 |
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| Author | Search for: Kartha, K. K.1; Search for: Gamborg, O. L.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | The cassava mosaic agent was eliminated from two cultivars of Indian and Nigerian origin by culturing shoot apical meristems on a Murashige and Skoog medium supplemented with benzyladenine (5 × 10⁻⁷ M), naphthaleneacetic acid (10⁻⁶ M) and gibberellic acid (GA3 10⁻⁷ M). Plant regeneration averaged 90 to 95% and 60% of the regenerated plants were mosaic symptom-free when explants of up to 0.4 mm in length were cultured. Growing diseased cuttings at 35 C for 30 days resulted in the production of plants without symptoms and from such plants meristem tips of up to 0.8 mm were cultured and mosaic disease-free plants regenerated. Results of graft transmission experiments demonstrated the absence of the causal agent in the plants regenerated by meristem culture technique. |
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| Publication date | 1975 |
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| Publisher | APS net |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | fc1e64ff-2063-48c1-941d-facbe2988429 |
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| Record created | 2023-07-27 |
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| Record modified | 2023-07-27 |
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