| DOI | Resolve DOI: https://doi.org/10.1073/pnas.1214809110 |
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| Author | Search for: Kuss, S.; Search for: Polcari, D.; Search for: Geissler, M.1; Search for: Brassard, D.1; Search for: Mauzeroll, J. |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | ferrocene derivative; ferrocenemethanol; multidrug resistance protein 1; ruthenium complex; unclassified drug; cancer cell; cell culture; cell structure; electrochemistry; female; HeLa cell; human cell; kinetics; multidrug resistance; protein localization; quantitative analysis; scanning electrochemical microscopy; target cell; uterine cervix cancer; HeLa cells; microelectrode; MRP1; Cell Culture Techniques; Drug Resistance, Multiple; Female; Ferrous Compounds; HeLa Cells; Microelectrodes; Microscopy, Fluorescence; Microscopy, Scanning Probe; Multidrug Resistance-Associated Proteins |
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| Abstract | The emergence of resistance to multiple unrelated chemotherapeutic drugs impedes the treatment of several cancers. Although the involvement of ATP-binding cassette transporters has long been known, there is no in situ method capable of tracking this transporter- related resistance at the single-cell level without interfering with the cell's environment or metabolism. Here, we demonstrate that scanning electrochemical microscopy (SECM) can quantitatively and noninvasively track multidrug resistance-related protein 1- dependent multidrug resistance in patterned adenocarcinoma cervical cancer cells. Nonresistant human cancer cells and their multidrug resistant variants are arranged in a side-by-side format using a stencil-based patterning scheme, allowing for precise positioning of target cells underneath the SECM sensor. SECM measurements of the patterned cells, performed with ferrocenemethanol and [Ru(NH3)6]3+ serving as electrochemical indicators, are used to establish a kinetic "map" of constant-height SECM scans, free of topography contributions. The concept underlying the work described herein may help evaluate the effectiveness of treatment administration strategies targeting reduced drug efflux. |
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| Publication date | 2013 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270567 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | fcc2b639-e891-4e35-bed1-4f7e76890995 |
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| Record created | 2014-02-17 |
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| Record modified | 2020-04-22 |
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