| Download | - View final version: Serum microRNA profiling to distinguish papillary thyroid cancer from benign thyroid masses (PDF, 1.4 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1186/s40463-015-0083-5 |
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| Author | Search for: Graham, M. Elise R.; Search for: Hart, Robert D.; Search for: Douglas, Susan1; Search for: Makki, Fawaz M.; Search for: Pinto, Devanand1ORCID identifier: https://orcid.org/0000-0002-0665-7041; Search for: Butler, Angela L.; Search for: Bullock, Martin; Search for: Rigby, Matthew H.; Search for: Trites, Jonathan R. B.; Search for: Taylor, S. Mark; Search for: Singh, Rama1 |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Funder | Search for: Dalhousie Department of Surgery Seed Grant; Search for: National Research Council of Canada |
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| Format | Text, Article |
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| Subject | papillary thyroid cancer; microRNA; serum testing; biomarkers |
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| Abstract | Objectives: Papillary thyroid cancer (PTC) is increasing in incidence. Fine needle aspiration is the gold standard for diagnosis, but results can be indeterminate. Identifying tissue and serum biomarkers, like microRNA, is therefore desirable. We sought to identify miRNA that is differentially expressed in the serum of patients with PTC.
Methods: Serum miRNA was quantified in 31 female thyroidectomy patients: 13 with benign disease and 18 with PTC. qPCR results were compared for significant fold-changes in 175 miRNAs, against a pooled control.
Results: 128 miRNA qualified for analysis. There were identifiable fold-changes in miRNA levels between benign and control, and between PTC and control. There were statistically significant fold changes in the level of four miRNAs between benign and PTC: hsa-miR-146a-5p and hsa-miR-199b-3p were down-regulated, while hsa-let7b-5p and hsa-miR-10a-5p were up-regulated.
Conclusions: MicroRNA is differentially expressed in the serum of patients with PTC. Serum miRNA has the potential to aid in thyroid cancer diagnosis. |
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| Publication date | 2015-09-05 |
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| Publisher | Sage |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | ffbbc684-41ef-4dc2-93d7-5ceee67b3d6c |
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| Record created | 2024-06-26 |
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| Record modified | 2024-06-26 |
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