Cellodextrin utilization and β-glucosidase production by Bacteroides polypragmatus

DOI	Trouver le DOI : https://doi.org/10.1007/BF00413033
Auteur	Rechercher : MacKenzie, Colin¹; Rechercher : Patel, G.¹
Affiliation	Conseil national de recherches du Canada
Format	Texte, Article
Résumé	Bacteroides polypragmatus, a mesophilic obligate anaerobe, was shown to simultaneously ferment glucose and cellobiose giving ethanol as a major metabolic end-product. A mixture of higher cellodextrins was also utilized. The bacterium produced a β-glucosidase with a pI value of 4.2 and a molecular weight of approximately 100000 daltons. The enzyme was intracellular and functioned optimally at pH 7. The K m values obtained with p-nitrophenyl-β-d-glucoside and cellobiose as substrates were 0.73 mM and 100 mM, respectively. The enzyme was quite stable at elevated temperatures; in the presence of 10% glycerol (v/v), it had a half-life of 4 h at 55°C. It was also stable during long-term storage at either 4°C or-20°C, provided that 10% (v/v) glycerol was added to preparations maintained at-20°C.
Date de publication	1986
Dans	Archives of Microbiology 145 (1986) : 91–96.
Langue	anglais
Numéro du CNRC	MACKENZIE1986 NRCC 25676
Numéro NPARC	9387828
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	01603cfa-0188-47f4-bf74-d9d916eabb13
Enregistrement créé	2009-07-10
Enregistrement modifié	2020-03-17