Laser-Induced Noninvasive Vascular Injury Models in Mice Generate Platelet- and Coagulation-dependent Thrombi

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1016/S0002-9440(10)64117-X
Auteur	Rechercher : Rosen, Elliot. D.; Rechercher : Raymond, Sylvain; Rechercher : Zollman, Amy; Rechercher : Noria, Francisco; Rechercher : Sandoval-Cooper, Mayra; Rechercher : Shulman, Alexis; Rechercher : Merz, James L.; Rechercher : Castellino, Francis J.
Format	Texte, Article
Résumé	A minimally invasive laser-induced injury model is described to study thrombus development in mice in vivo. The protocol involves focusing the beam of an argon-ion laser through a compound microscope on the vasculature of a mouse ear that is sufficiently thin such that blood flow can be visualized by intravital microscopy. Two distinct injury models have been established. The first involves direct laser illumination with a short, high-intensity pulse. In this case, thrombus formation is inhibited by the GPIIb/IIIa antagonist, G4120. However, the anticoagulants, hirulog, PPACK, and NapC2 have minimal effect. This indicates that thrombus development induced by this model mainly involves platelet interactions. The second model involves low-intensity laser illumination of mice injected with Rose Bengal dye to induce photochemical injury in the region of laser illumination. Thrombi generated by this latter procedure have a slower development and are inhibited by both anticoagulant and anti-platelet compounds.
Date de publication	2001-05-01
Maison d’édition	Elsevier
Dans	American Journal of Pathology 158 : 1613–1622.
Publication du CNRC	Cette publication n’est pas du CNRC Ce sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC.
Numéro NPARC	12743879
Exporter la notice	Exporter en format RIS
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Identificateur de l’enregistrement	05526be3-8b8c-44a9-991a-7b39e9c1b8e0
Enregistrement créé	2009-10-27
Enregistrement modifié	2020-03-27

Date de modification :: 2024-07-18