| Auteur | Rechercher : Howard, Sarah L.; Rechercher : Jagannathan, Aparna; Rechercher : Soo, Evelyn C.1; Rechercher : Hui, Joseph P. M.1; Rechercher : Aubry, Annie J.2; Rechercher : Ahmed, Imran; Rechercher : Karlyshev, Andrey; Rechercher : Kelly, John F.2; Rechercher : Jones, Michael A.; Rechercher : Stevens, Mark P.; Rechercher : Logan, Susan M.2; Rechercher : Wren, Brendan W. |
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| Affiliation | - Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | acid; biofilms; biosynthesis; campylobacter; campylobacter jejuni; cell; chain; chickens; flagellin; form; genes; glycosylation; mechanisms; molecular; mutagenesis; mutant; protein; strain; structural-analysis; surface; veterinary |
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| Résumé | Previously we identified five genes (Cj1321-26) in the O-linked flagellin glycosylation island that were highly prevalent in Campylobacter jejuni isolates from chickens. We report mutagenesis, functional and structural data to confirm that this locus, and Cj1324 in particular, has a significant contributory role in the colonisation of C. jejuni in chickens. A motile DeltaCj1324 mutant with intact flagellae was considerably less hydrophobic and less able to autoagglutinate and form biofilms than the parent strain 11168H, suggesting that the surface charge of flagellae was altered in Cj1324-deficient strains. These physical and functional attributes were restored upon complementation. Structural analysis of flagellin protein purified from the DeltaCj1324 mutant revealed the absence of two legionaminic acid glycan modifications that were present in the parent strain 11168H. These glycoform modifications were shown to be prevalent in chicken isolates and confirm that differences in the highly variable flagellin glycosylation locus can relate to strain source. The discovery of molecular mechanisms influencing persistence of C. jejuni in poultry aids the rational design of approaches to control this problematic pathogen in the food chain |
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| Date de publication | 2009-03-23 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | HOWARD2009 |
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| Numéro NPARC | 9381698 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 07a525a1-6b0e-4e76-be88-a9e00df47636 |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-04-16 |
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