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Development of a fast extraction method and optimization of liquid chromatography–mass spectrometry for the analysis of phenolic compounds in lentil seed coats

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/j.jchromb.2014.08.007
AuteurRechercher : ; Rechercher : 1; Rechercher : 1; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
FormatTexte, Article
Sujetacetone; chromatography; extraction; flavonoids; high performance liquid chromatography; mass spectrometry; phenols; solvents; chromatographic conditions; condition optimizations; core-shell columns; lens culinaris; liquid chromatography; mass spectrometries; liquid chromatography-mass spectrometry; organic compositions; polyphenols; drug isolation; drug screening; flow rate; genotype; lentil; plant breeding; seed husk; ultraviolet radiation
Résumé
A systematic set of optimization experiments was conducted to design an efficient extraction and analysis protocol for screening six different sub-classes of phenolic compounds in the seed coat of various lentil (Lens culinaris Medik.) genotypes. Different compounds from anthocyanidins, flavan-3-ols, proanthocyanidins, flavanones, flavones, and flavonols sub-classes were first optimized for use as standards for liquid chromatography mass spectrometry (LC-MS) with UV detection. The effect of maceration duration, reconstitution solvent, and extraction solvent were investigated using lentil genotype CDC Maxim. Chromatographic conditions were optimized by examining column separation efficiencies, organic composition, and solvent gradient. The results showed that a 1. h maceration step was sufficient and that non-acidified solvents were more appropriate; a 70:30 acetone: water (v/v) solvent was ultimately selected. Using a Kinetex PFP column, the organic concentration, gradient, and flow rate were optimized to maximize the resolution of phenolic compounds in a short 30-min analysis time. The optimized method was applied to three lentil genotypes with different phenolic compound profiles to provide information of value to breeding programs.
Date de publication
Dans
  • Journal of Chromatography B 969 (12 août 2014) : 149–161.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRC-ACRD-56082
Numéro NPARC21273872
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement0b319d3c-ec03-47dc-b84d-0662d6b9db5b
Enregistrement créé2015-01-30
Enregistrement modifié2020-04-22
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