| Autre titre | Application of UPLC-QTOF-MS in MSE mode for the rapid and precise identification of alkaloids in goldenseal (Hydrastis canadensis) |
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| Téléchargement | - Voir le manuscrit accepté : Separation and characterization of alkaloids in goldenseal (Hydrastis canadensis) by ultra-performance liquid chromatography coupled with electrospray ionization quadruple time of flight mass spectrometry (UPLC/Q-TOF-MS) (PDF, 1.9 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1007/s00216-013-7558-x |
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| Auteur | Rechercher : Le, P.M.1; Rechercher : McCooeye, M.1; Rechercher : Windust, A.1 |
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| Affiliation | - Conseil national de recherches du Canada. Science des mesures et étalons
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| Format | Texte, Article |
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| Résumé | Here, we describe a new application of ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MSE mode (UPLC-QTOF-MSE) for the sensitive, fast, and effective characterization of alkaloids in goldenseal (Hydrastis canadensis). This approach allowed identification of alkaloids using a cyclic low and high collision energy spectral acquisition mode providing simultaneous accurate precursor and fragment ion mass information. A total of 45 compounds were separated and 40 of them characterized including one new compound and 7 identified for the first time in goldenseal. The spectral data obtained using this method is comparable to those obtained by conventional LC-MSn. However, the UPLC-QTOF-MSE method offers high chromatographic resolution with structural characterization facilitated by accurate mass measurement in both MS and MS/MS modes in a single analytical run; this makes it suitable for the rapid analysis and screening of alkaloids in plant extracts. [Figure not available: see fulltext.] © 2014 Her Majesty the Queen in Right of Canada. |
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| Date de publication | 2014 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21270802 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 0caece70-6f53-4350-bf02-8e7625264f7a |
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| Enregistrement créé | 2014-02-17 |
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| Enregistrement modifié | 2020-06-04 |
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