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Oxidative metabolism and protoplast culture

DOITrouver le DOI : https://doi.org/10.1007/BF02632035
AuteurRechercher : ; Rechercher : ; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Sujetbarley; protoplast; oxidative stress; glutathione
Résumé
Barley leaf blade protoplasts accumulate malonaldehyde, a product of lipid peroxidation, during culture. In addition, glutathione levels fall after protoplast isolation and the proportion of glutathione in the oxidized state rises. These data indicate oxidative stress after protoplast isolation and during culture. The cause of this phenomenon is revealed by data showing that the activities of enzymes associated with antioxidative processes including glutathione reductase and ascorbate peroxidase decrease after barley protoplast isolation. In contrast, protoplasts isolated from suspension cultured cells of bromegrass and soybean exhibit little evidence for oxidative stress and increased activities of glutathione reductase and ascorbate peroxidase. We suggest that an antioxidative response is associated with mitosis and colony formation from protoplasts, as exhibited by bromegrass and soybean. Conversely, failure of an antioxidative response is associated with low viability and absence of mitosis, as in barley. Increased viability of barley leaf protoplasts cultured on feeder layer cells is correlated with increased glutathione content and higher glutathione reductase activity.
Date de publication
Maison d’éditionSpringer Nature
Dans
  • In Vitro Cellular & Developmental Biology - Plant 29, nº 4 (octobre 1993) : 200–206.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurBF02632035
NRCC-36486
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement11c91c91-d343-4ef0-b76b-483abbca53e0
Enregistrement créé2023-11-02
Enregistrement modifié2023-11-02

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