| DOI | Trouver le DOI : https://doi.org/10.1515/hsz-2012-0142 |
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| Auteur | Rechercher : Fischer, K.; Rechercher : Vinogradov, E.1; Rechercher : Lindner, B.; Rechercher : Heine, H.; Rechercher : Holst, O. |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | antiallergic agent; extracellular teichoic acid; sodium chloride; teichoic acid; unclassified drug; allergic asthma; chemical structure; electrospray mass spectrometry; extraction; gel permeation chromatography; ion cyclotron resonance mass spectrometry; Lactococcus lactis; proton nuclear magnetic resonance; Carbohydrate Sequence; Hypersensitivity; Lactococcus lactis; Mice; Molecular Sequence Data; Nuclear Magnetic Resonance, Biomolecular; Spectrometry, Mass, Electrospray Ionization; Teichoic Acids |
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| Résumé | The Gram-positive bacterium Lactococcus lactis G121 is a farm isolate that protects mice from ovalbumin-induced asthma. To understand the molecular mechanisms of such allergy-protective properties, the isolation and characterization of cell envelope constituents is crucial. Here, structural analyses of the extracellular teichoic acid (EC TA) from L. lactis G121 are presented. Extraction with 0.9 % saline afforded a crude TA fraction. Consecutive size exclusion chromatography on Biogel P60 and P10 matrix was performed to purify the sample. Chemical component analyses, high-resolution electrospray ionization Fourier-transformed ion cyclotron mass spectrometry, and nuclear magnetic resonance spectroscopy were conducted for structural elucidation. The EC TA was a poly(glycosylglycerol phosphate) molecule with a repeating unit of -6)-[β- d-Glcp -(1→3)-][α- d-Glcp NAc-(1→4)-]α- d-Galp NAc-(1→3)-β-d-Glcp NAc-(1→2)-glycerol-(1- P -). Copyright © by Walter de Gruyter • Berlin • Boston. |
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| Date de publication | 2012 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21269410 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 1463305d-cd2d-4694-97d6-5be431f56e7f |
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| Enregistrement créé | 2013-12-12 |
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| Enregistrement modifié | 2020-04-21 |
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