| DOI | Trouver le DOI : https://doi.org/10.1016/j.chroma.2007.09.031 |
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| Auteur | Rechercher : Rowe, Gerald E.1; Rechercher : Aomari, Hafida1; Rechercher : Chevaldina, Tatiana1; Rechercher : Lafrance, Martin1; Rechercher : St-Arnaud, Sylvie1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | Acids; Adsorption; Amino Acids; analogs & derivatives; Biotechnology; chemistry; Chromatography; Chromatography,Liquid; Esters; Heat; Hydrogen Bonding; Hydrophobicity; methods; Models,Chemical; Polymers; Sepharose; Solubility; Thermodynamics; Hydrophobic interaction chromoatography; N-Acetyl amino acid methyl esters; biotechnology; Temperature |
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| Résumé | Thermodynamic analysis of hydrophobic interaction chromatography of amino acid methyl esters showed entropy-driven adsorption, consistent with solvophobic theory, except for phenyl ester on the Toyopearl resins. All esters adsorbed more strongly to the Toyopearl resins, including the polymethacrylate base matrix, than to Butyl Sepharose. Enthalpy changes were more favorable with the former, explaining the retention difference between Toyopearl Butyl and Butyl Sepharose. An enthalpy change versus heat capacity change plot showed Van derWaals interactions predominantly with the resin matrix. Literature data revealed the same effect for dansylamino acids, shown by isothermodynamic temperature analysis to adsorb more entropically than the esters. |
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| Date de publication | 2007-09-18 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | NRCC 52722 |
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| Numéro NPARC | 12401003 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 181b34cb-8fde-4b2a-a05c-796d146f53a7 |
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| Enregistrement créé | 2009-10-26 |
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| Enregistrement modifié | 2020-05-10 |
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