| DOI | Trouver le DOI : https://doi.org/10.1007/BF00384898 |
|---|
| Auteur | Rechercher : Fowke, L. C.; Rechercher : Constabel, F.1; Rechercher : Gamborg, O. L.1 |
|---|
| Affiliation | - Conseil national de recherches du Canada. Laboratoire régional des prairies
|
|---|
| Format | Texte, Article |
|---|
| Sujet | cell culture; glycine; pisum; protoplasts fusion |
|---|
| Résumé | Protoplasts from pea (Pisum sativum L.) leaves and cultured soybean (Glycine max L.) cells were fused by means of polyethylene glycol and subsequently cultured for one week. Both agglutinated protoplasts and cultured fusion products were examined by electron microscopy. Agglutination occurred over large areas of the plasma membranes. The membrane contanct was discontinuous and irregularly spaced. Many cultured fusion products regenerated cell walls and divided to form cell clusters. Fusion of pea and soybean interphase nuclei occurred in some cells. The detection of heterochromatin typical of pea in the synkaryon, even after division, suggests the cells were hybrids. The cytoplasm of the cells from the fusion products contained both soybean leucoplasts and pea chloroplasts. The chloroplasts had apparently ceased dividing and some showed signs of degenerating. Large multinucleate fusion products developed cell walls but failed to divide. |
|---|
| Date de publication | 1977-01 |
|---|
| Maison d’édition | Springer Nature |
|---|
| Dans | |
|---|
| Langue | anglais |
|---|
| Publications évaluées par des pairs | Oui |
|---|
| Identificateur | BF00384898 |
|---|
| Exporter la notice | Exporter en format RIS |
|---|
| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
|---|
| Identificateur de l’enregistrement | 1be22a6a-dfd4-4fda-9a29-0e60f673a516 |
|---|
| Enregistrement créé | 2023-07-06 |
|---|
| Enregistrement modifié | 2023-07-11 |
|---|
