| Téléchargement | - Voir la version finale : JadX is a disparate natural product binding protein (PDF, 2.1 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1021/jacs.5b11286 |
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| Auteur | Rechercher : Robertson, Andrew W.; Rechercher : Forget, Stephanie M.; Rechercher : Martinez-Farina, Camilo F.; Rechercher : McCormick, Nicole E.; Rechercher : Syvitski, Raymond T.1; Rechercher : Jakeman, David L. |
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| Affiliation du nom | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Article |
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| Résumé | We report that JadX, a protein of previously undetermined function coded for in the jadomycin biosynthetic gene cluster of Streptomyces venezuelae ISP5230, affects both chloramphenicol and jadomycin production levels in blocked mutants. Characterization of recombinant JadX through protein–ligand interactions by chemical shift perturbation and WaterLOGSY NMR spectroscopy resulted in the observation of binding between JadX and a series of jadomycins and between JadX and chloramphenicol, another natural product produced by S. venezuelae ISP5230. These results suggest JadX to be an unusual class of natural product binding protein involved in binding structurally disparate natural products. The ability for JadX to bind two different natural products in vitro and the ability to affect production of these secondary metabolites in vivo suggest a potential role in regulation or signaling. This is the first example of functional characterization of these JadX-like proteins, and provides insight into a previously unobserved regulatory process. |
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| Date de publication | 2016-01-27 |
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| Maison d’édition | ACS Publications |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 23000138 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 1cef32c4-4c83-4b35-af83-2602ff6d1af3 |
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| Enregistrement créé | 2016-06-07 |
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| Enregistrement modifié | 2020-06-02 |
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