| Téléchargement | - Voir la version finale : Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid (PDF, 1.5 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1021/bi00020a019 |
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| Auteur | Rechercher : Evans, S.1; Rechercher : Sigurskjold, B. W.1; Rechercher : Jennings, H. J.1; Rechercher : Brisson, J.-R.1; Rechercher : To, R.1; Rechercher : Tse, W. C.1; Rechercher : Altman, Eleonora1; Rechercher : Frosch, M.; Rechercher : Weisgerber, C.1; Rechercher : Kratzin, H. D.; Rechercher : Klebert, S.; Rechercher : Vaesen, M.; Rechercher : Bitter-Suermann, D.; Rechercher : Rose, D. R.1; Rechercher : Young, N. M.1; Rechercher : Bundle, D. R.1 |
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| Affiliation | - Conseil national de recherches Canada
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| Format | Texte, Article |
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| Résumé | The antigen binding fragment from an IgG2a kappa murine monoclonal antibody with specificity for alpha-(2-->8)-linked sialic acid polymers has been prepared and crystallized in the absence of hapten. Crystals were grown by vapor diffusion equilibrium with 16-18% polyethylene glycol 4000 solutions. The structure was solved by molecular replacement methods and refined to a conventional R factor of 0.164 for data to 2.8 A. The binding site is observed to display a shape and distribution of charges that is complementary to that of the predicted conformation of the oligosaccharide epitope. A thermodynamic description of ligand binding has been compiled for oligosaccharides ranging in length from 9 to 41 residues, and the data for the largest ligand has been used in a novel way to estimate the size of the antigen binding site. A model of antigen binding is presented that satisfies this thermodynamic data, as well as a previously reported requirement of conformational specificity of the oligosaccharide. X-ray crystallographic and thermodynamic evidence are consistent with a binding site that accommodates at least eight sialic acid residues |
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| Date de publication | 1995-05-23 |
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| Maison d’édition | American Chemical Society |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | EVANS1995 |
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| Numéro NPARC | 9371205 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 1df6fea1-59aa-4dbc-90d2-3463988cd415 |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-05-27 |
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