| DOI | Trouver le DOI : https://doi.org/10.1007/s00216-011-4934-2 |
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| Auteur | Rechercher : Roa, Wilson; Rechercher : Yang, Xiaoyan; Rechercher : Guo, Linghong; Rechercher : Huang, Biao; Rechercher : Khatibisepehr, Shima; Rechercher : Gabos, Stephan; Rechercher : Chen, Jie1; Rechercher : Xing, James |
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| Affiliation | - Conseil national de recherches du Canada. Institut national de nanotechnologie
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| Format | Texte, Article |
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| Sujet | Cancer radiotherapy; Cell radiosensitivity; Clonogenic assays; Gold nanoparticle; RT-CES cell-proliferation assay; Radiotherapy; cell survival; neoplasm; pathology; radiation dose; treatment outcome; tumor cell line; Neoplasms; Radiation Tolerance |
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| Résumé | Intrinsic radiosensitivity of normal and tumour tissues has been shown to be an independent prognostic factor for patients' response to radiotherapy. This study compares the real-time cell-impedance sensing (RT-CES) assay with the conventional clonogenic assay in terms of in-vitro radiosensitivity. One objective in this study was to predict in-vivo response to gold nanoparticle (GNP) treatment on the basis of in-vitro RT-CES testing results. Four adenocarcinoma cancer cell lines were tested using both the RT-CES and the clonogenic assays. Cell-survival curves were plotted, and the mean SF2 values obtained by these two different assay methods were compared using ANOVA. Radiation sensitivities obtained in-vitro were then compared with the in-vivo results. On the basis of the measurement of cell colonies, the RT-CES assay has similar radiosensitivity to the clonogenic assay, but significantly shortens the testing time from 14-21 days to only 72 h. Intrinsic GNP enhanced radiation sensitivity using tumour volume (mm3) in vivo is comparable with that using RT-CES cell survival assay in vitro. Furthermore, the RT-CES system provides real-time information regarding the state of cell radiosensitivity that may give useful information towards personalizing radiotherapy. The RT-CES assay enables more reliable and time-efficient results in the evaluation of radiosensitivity. [Figure not available: see fulltext.] © 2011 Springer-Verlag. |
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| Date de publication | 2011-06-01 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 21272017 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 2172f83c-3e06-434c-967a-4d609f058440 |
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| Enregistrement créé | 2014-05-21 |
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| Enregistrement modifié | 2020-04-21 |
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