| Autre titre | Immunological responses of turbot (Psetta maxima) to nodavirus or poly I:C stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays |
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| DOI | Trouver le DOI : https://doi.org/10.1016/j.fsi.2008.03.010 |
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| Auteur | Rechercher : Park, Kyoung C.1; Rechercher : Osborne, Jane A.1; Rechercher : Montes, Ariana; Rechercher : Dios, Sonia; Rechercher : Nerland, Audund H.; Rechercher : Novoa, Beatriz; Rechercher : Figueras, Antonio; Rechercher : Brown, Laura L.; Rechercher : Johnson, Stewart C. |
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| Affiliation | - Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
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| Format | Texte, Article |
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| Sujet | Turbot; Nodavirus; pIC; infection; immune; EST; microarrays |
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| Résumé | To investigate the immunological responses of turbot to nodavirus infection or pIC stimulation, we constructed cDNA libraries from liver, kidney and gill tissues of nodavirus-infected fish and examined the differential gene expression within turbot kidney in response to nodavirus infection or pIC stimulation using a turbot cDNA microarray. Turbot were experimentally infected with nodavirus and samples of each tissue were collected at selected time points post-infection. Using equal amount of total RNA at each sampling time, we made three tissue-specific cDNA libraries. After sequencing 3230 clones we obtained 3173 (98.2%) high quality sequences from our liver, kidney and gill libraries. Of these 2568 (80.9%) were identified as known genes and 605 (19.1%) as unknown genes. A total of 768 unique genes were identified. The two largest groups resulting from the classification of ESTs according to function were the cell/organism defense genes (71 uni-genes) and apoptosis-related process (23 uni-genes). Using these clones, a 1920 element cDNA microarray was constructed and used to investigate the differential gene expression within turbot in response to experimental nodavirus infection or pIC stimulation. Kidney tissue was collected at selected times post-infection (HPI) or stimulation (HPS), and total RNA was isolated for microarray analysis. Of the 1920 genes studied on the microarray, we identified a total of 121 differentially expressed genes in the kidney: 94 genes from nodavirus-infected animals and 79 genes from those stimulated with pIC. Within the nodavirus-infected fish we observed the highest number of differentially expressed genes at 24 HPI. Our results indicate that certain genes in turbot have important roles in immune responses to nodavirus infection and dsRNA stimulation. |
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| Date de publication | 2008-03-26 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 42763
1726 |
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| Numéro NPARC | 3538382 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 21771f41-af04-46a0-843c-044832ef2f5e |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-04-15 |
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