| Résumé | A chemically defined medium for the cultivation of Valsa friesii Duby (Fuckel) PRL 1736 has been developed and conditions established for accumulation of high yields of m-cresol in the culture filtrate. Yields of 1 g per liter as determined by colorimetric assay using 4-aminoantipyrine are obtained after 10 days' incubation in shake culture at 28 °C in the absence of light on a medium containing glucose, 15 g; alanine, 2 g; K₂HPO₄, 0.3 g; KH₂PO₄, 0.2 g; salts (0.2 g MgSO₄∙7H₂O, 0.1 g NaCl, 0.1 g CaCl₂, 0.015 g FeSO₄, and 0.25 mg ZnCl₂); and vitamins (4 mg each of thiamine HCl, pyridoxin HCl, 10 mg inositol, and 10 μg biotin) per liter of distilled water. The organism is shown to tolerate higher concentrations of m-cresol than do eight other fungi tested. |
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