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Solution conformation of asparagine-linked oligosaccharides: α(1-2)-, α(1-3)-, β(1-2)-, and β(1-4)-linked units

Par Conseil national de recherches du Canada

Autre titreSolution conformation of asparagine-linked oligosaccharides: alpha(1-2)-, alpha(1-3)-, beta(1-2)-, and beta(1-4)-linked units
DOITrouver le DOI : https://doi.org/10.1021/bi00284a021
AuteurRechercher : ; Rechercher :
FormatTexte, Article
Sujet1H; alpha; asparagine; asparagine-linked oligosaccharides; calculation; carbohydrate conformation; carbohydrate sequence; chemical; chemical shift; conformation; conformational; energy; energy calculations; enhancement; glcnac; glycopeptide; high-resolution; kinetics; linkages; magnetic; magnetic resonance spectroscopy; models, molecular; molecule; NMR; nuclear; nuclear magnetic resonance; nuclear-magnetic-resonance; oligosaccharide; oligosaccharides; overhauser; overhauser enhancement; parameters; potential; residues; resonance; shifts; solution; solution conformation; structure-activity relationship; support, Non-U. S. Gov't; unit
Résumé
The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of α(1-3)-, α(1-2)-, β(1-2)-, and β(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolution ¹H nuclear magnetic resonance in conjunction with potential energy calculations. The NMR parameters that were used in this analysis were chemical shifts and nuclear Overhauser enhancements. Potential energy calculations were used to evaluate the preferred conformers available for the different linkages in glycopeptides and to draw conclusions about the behavior in solution of these molecules. It was found that the linkage conformation of the Man α 1-3 residues was not affected by substitution either at the 2-position by α Man or β GlcNAc or at the 4-position by beta GlcNAc or by the presence of a bisecting GlcNAc on the adjacent β Man residue
Date de publication
Maison d’éditionAmerican Chemical Society
Dans
  • Biochemistry 22, nº 15 : 3671–3680.
Langueanglais
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IdentificateurNRC-IBS-BRISSON1983G
Numéro NPARC9388957
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Identificateur de l’enregistrement265902d8-134d-4610-87cf-f21a447bdf6f
Enregistrement créé2009-07-10
Enregistrement modifié2020-03-13
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