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Gouvernement du Canada / Government of Canada

Recherche

Recombinant production and characterization of the carbohydrate recognition domain from Atlantic salmon C-type lectin receptor C (SCLRC)

Par Conseil national de recherches du Canada

AuteurRechercher : 1; Rechercher : 1; Rechercher : 2
Affiliation
  1. Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
  2. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
FormatTexte, Article
Résumé
The Atlantic salmon C-type lectin receptor C (SCLRC) locus encodes a potential oligomeric type II receptor. C-type lectins recognize carbohydrates in a Ca2+-dependent manner through structurally conserved, yet functionally diverse, C-type lectin-like domains (CTLDs). Many conserved amino acids in animal CTLDs are present in SCLRC, with the notable exception of an asparagine crucially involved in Ca2+- and carbohydrate-binding, which is tyrosine in SCLRC. SCLRC also contains six cysteines that form three disulfide bonds. Although SCLRC was originally identified as an up-regulated transcript responding to Aeromonas salmonicida infection, the biological role of this protein is still unknown. To study the structure and ligand binding properties of SCLRC, we created a homology model of the 17 kDa CTLD and produced it as an affinity-tagged protein in the periplasm of Escherichia coli by co-expression of proteins that facilitate disulfide bond formation. The recombinant form of SCLRC was characterized by a protease protection assay, a solid-phase carbohydrate-binding assay, and frontal affinity chromatography. On the basis of this characterization, we classify SCLRC as a C-type lectin that binds to mannose and its derivatives.
Date de publication
Dans
  • Protein Expression and Purification 59, nº 1 (mai 2008) : 38–46.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 42761
1724
Numéro NPARC3538214
Exporter la noticeExporter en format RIS
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Identificateur de l’enregistrement2952f037-d05e-44bd-b32b-31add8ec70bd
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-15
Date de modification :