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Gouvernement du Canada / Government of Canada

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Archaeosomes as novel antigen delivery systems

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1016/S0378-1097(97)00294-2
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
FormatTexte, Article
Sujetarchaeosome; archaeobacterial lipid; liposome; humoral immune response; albumin; cholera toxin; mice
Résumé
The humoral immune response mounted in BALB/c mice against bovine serum albumin or cholera toxin B subunit was compared when the antigens were associated with liposomes composed of either archaeal ether lipids or conventional lipids. Antibody titres in sera from mice immunised intraperitoneally were elevated to an extent comparable to those achieved with Freund's adjuvant by encapsulating bovine serum albumin in archaeal lipid vesicles (archaeosomes) of about 200 nm diameter. Comparison among six archaeosome and three conventional liposome compositions established that archaeosomes were generally much superior in potentiating an immune response. Further, only two immunisations, at the most, were needed to achieve close to the maximum antibody titre, as shown with archaeosomes composed of the polar lipids from Methanobrevibacter smithii, an inhabitant of the human colon. A similar positive response to presenting the more immunogenic cholera B subunit protein to the immune system of mice was shown for M. smithii archaeosomes. Encapsulation of the antigen in the archaeosome was necessary to achieve the full humoral response. This represents the first study to our knowledge where archaeosomes have been evaluated in vivo as possible antigen carriers.
Date de publication
Dans
  • FEMS Microbiology Letters 154 (1997) : 17–22.
Langueanglais
Numéro du CNRCSPROTT1997A
NRCC 39542
Numéro NPARC9363550
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Identificateur de l’enregistrement2ebad4a8-2494-4678-9438-fa651b852609
Enregistrement créé2009-07-10
Enregistrement modifié2020-03-20
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