Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1039/b409366f
Auteur	Rechercher : Furdui, Vasile I.; Rechercher : Harrison, D. Jed
Format	Texte, Article
Résumé	A one-step immunomagnetic separation technique was performed on a microfluidic platform for the isolation of specific cells from blood samples. The cell isolation and purification studies targeted T cells, as a model for low abundance cells (about 1[ratio]10,000 cells), with more dilute cells as the ultimate goal. T cells were successfully separated on-chip from human blood and from reconstituted blood samples. Quantitative polymerase chain reaction analysis of the captured cells was used to characterize the efficiency of T cell capture in a variety of flow path designs. Employing many (4-8), 50 [micro sign]m deep narrow channels, with the same overall cross section as a single, 3 mm wide channel, was much more effective in structuring dense enough magnetic bead beds to trap cells in a flowing stream. The use of 8-multiple bifurcated flow paths increased capture efficiencies from [similar]20 up to 37%, when compared to a straight 8-way split design, indicating the value of ensuring uniform flow distribution into each channel in a flow manifold for effective cell capture. Sample flow rates of up to 3 [micro sign]L min-1 were evaluated in these capture beds.
Date de publication	2004
Dans	Lab on a Chip 4, nº 6 (2004) : 614–618.
Publication du CNRC	Cette publication n’est pas du CNRC Ce sont des publications qui ont été rédigées par un auteur du CNRC, mais avant que celui-ci soit employé du CNRC.
Numéro NPARC	12333684
Exporter la notice	Exporter en format RIS
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Identificateur de l’enregistrement	2fac873f-70ce-4365-8693-6ffc6aa4e6fa
Enregistrement créé	2009-09-10
Enregistrement modifié	2020-04-17

Date de modification :: 2024-07-05