Promoter sequences from two different Brassica napus tapetal oleosin-like genes direct tapetal expression of ß-glucuronidase in transgenic Brassica plants

Par Conseil national de recherches du Canada

DOI	Trouver le DOI : https://doi.org/10.1023/A:1005840824926
Auteur	Rechercher : Ping Hong, Hai¹; Rechercher : Ross, Joanne H.E.; Rechercher : Gerster, Jean L.; Rechercher : Rigas, Stamatis; Rechercher : Datla, Raju S.S.¹; Rechercher : Hatzopoulos, Polydefkis; Rechercher : Scoles, Graham; Rechercher : Keller, Wilf¹; Rechercher : Murphy, Denis J.; Rechercher : Robert, Laurian S.
Affiliation	Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
Format	Texte, Article
Sujet	anther; Brassica; ß-glucuronidase; oleosin; promoter; tapetum
Résumé	To investigate the sequences responsible for the regulated expression of tapetal-specific oleosin-like genes, ca. 2 kb of the 5′-upstream regions from two divergent genes, OlnB;4 and OlnB;13, were isolated, sequenced and fused to the reporter gene β-glucuronidase for study in transgenic Brassica napus plants. Although the proteins encoded by these two genes are highly divergent, except for the conserved oleosin-like domain, the first 250 bp of their 5′-upstream regions was 86% identical, including a region of 150 bp upstream from the TATA box. Analysis of 42 independent transformants by histochemical and fluorometric methods showed that both promoters directed tapetal-specific expression that peaked at the 4 mm flower bud stage.
Date de publication	1997-06
Maison d’édition	Springer Link
Dans	Plant Molecular Biology 34, nº 3 (juin 1997) : 549–555.
Langue	anglais
Publications évaluées par des pairs	Oui
Identificateur	138684
Exporter la notice	Exporter en format RIS
Signaler une correction	Signaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement	30c707ce-74ae-4676-920c-52ca6ab5c63e
Enregistrement créé	2023-11-01
Enregistrement modifié	2023-11-01

Date de modification :: 2024-06-30