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Ion suppression: a major concern in mass spectrometry

AuteurRechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches Canada. Institut des biosciences marines du CNRC
FormatTexte, Article
Sujetmass spectrometry; ion suppression
Résumé
Ion suppression is one form of matrix effect that liquid chromatography–mass spectrometry (LC–MS) techniques suffer from, regardless of the sensitivity or selectivity of the mass analyzer used. Ion suppression negatively affects several analytical figures of merit, such as detection capability, precision, and accuracy. The limited knowledge of the origin and mechanism of ion suppression makes this problem difficult to solve in many cases. Over the past decade and a half since the response-reducing phenomenon was exposed, however, protocols have been developed not only to test for its presence but also to account for its effects and eliminate the risk of ion suppression altogether. Because there is no universal solution for the matrix effect, some of the viable options are discussed briefly in this tutorial, which alone or in combination can help regain the quality of LC–MS analysis for the particular matrix–analyte combination. Two commonly used techniques to detect the presence of the matrix effect are illustrated. Modifying instrumental components and parameters, chromatographic separation, and sample preparation are all considered as means of reducing or possibly eliminating ion suppression. A variety of calibration techniques for compensating the effects of the phenomenon also are discussed.
Date de publication
Maison d’éditionUBM Life Sciences
Dans
  • LCGC North America 24, nº 5 : 498–510.
Langueanglais
Publications évaluées par des pairsNon
Numéro du CNRCNRCC 55859
1420
Numéro NPARC3538339
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement30ce0d9d-7e0a-43d4-bd2d-9022430a67b2
Enregistrement créé2009-03-01
Enregistrement modifié2020-04-22

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