Sélection de la langue

Gouvernement du Canada / Government of Canada

Recherche

Comparison of the 5.8s rDNA and internal transcribed spacer sequences of isolates of Leptosphaeria maculans from different pathogenicity groups

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/BF00312640
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Sujetblackleg; phytopathogen; ribosomal RNA; Leptosphaeria
Résumé
The regions coding for the 5.8s rRNA and the flanking internal transcribed spacers (ITS1 and ITS2) from nine isolates of the blackleg pathogen Leptosphaeria maculans and one isolate of Sclerotinia sclerotiorum were amplified by the polymerase chain reaction and sequenced. Five of the L. maculans isolates were highly virulent to Brassica plants, two were weakly virulent and two were isolated from the cruciferous weed Thlaspi arvense. The 5.8s DNA sequences of all L. maculans isolates were identical. However, there were major differences in both ITS1 and ITS2 sequences that correlated with the pathogenicity grouping. Phylogenetic analysis of the ITS sequences by both parsimony and maximum-likelihood methods indicated that each pathogenicity group was statistically different from each other with the weaklyvirulent isolates being more closely related to the Thlaspi than to the highly-virulent isolates. The relationships of L. maculans to other fungi, based on a comparison of the 5.8s rDNA sequences, are discussed.
Date de publication
Maison d’éditionSpringer Nature
Dans
  • Current Genetics 23, nº 5-6 (1993) : 490–495.
Langueanglais
Publications évaluées par des pairsOui
IdentificateurBF00312640
NRCC-35120
Exporter la noticeExporter en format RIS
Signaler une correctionSignaler une correction (s'ouvre dans un nouvel onglet)
Identificateur de l’enregistrement3510ebc4-1ac0-4662-9f43-1177e7eb4901
Enregistrement créé2023-11-06
Enregistrement modifié2023-11-06
Date de modification :