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Isolation of a cDNA encoding a granule-bound 152-kilodalton starch-branching enzyme in wheat

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1104/pp.124.1.253
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1; Rechercher : ; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut de biotechnologie des plantes du CNRC
FormatTexte, Article
Résumé
Screening of a wheat (Triticum aestivum) cDNA library for starch-branching enzyme I (SBEI) genes combined with 5′-rapid amplification of cDNA ends resulted in isolation of a 4,563-bp composite cDNA, Sbe1c. Based on sequence alignment to characterized SBEI cDNA clones isolated from plants, the SBEIc predicted from the cDNA sequence was produced with a transit peptide directing the polypeptide into plastids. Furthermore, the predicted mature form of SBEIc was much larger (152 kD) than previously characterized plant SBEI (80–100 kD) and contained a partial duplication of SBEI sequences. The first SBEI domain showed high amino acid similarity to a 74-kD wheat SBEI-like protein that is inactive as a branching enzyme when expressed in Escherichia coli. The second SBEI domain on SBEIc was identical in sequence to a functional 87-kD SBEI produced in the wheat endosperm. Immunoblot analysis of proteins produced in developing wheat kernels demonstrated that the 152-kD SBEIc was, in contrast to the 87- to 88-kD SBEI, preferentially associated with the starch granules. Proteins similar in size and recognized by wheat SBEI antibodies were also present inTriticum monococcum, Triticum tauschii, and Triticum turgidum subsp. durum.
Date de publication
Maison d’éditionOxford Academic
American Society of Plant Biologists
Dans
  • Plant Physiology 124, nº 1 (1 septembre 2000) : 253–262.
Langueanglais
Publications évaluées par des pairsOui
Numéro NPARC13053880
Exporter la noticeExporter en format RIS
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Identificateur de l’enregistrement37c316b3-703a-43ba-a859-334b6b029516
Enregistrement créé2009-11-25
Enregistrement modifié2023-04-13
Date de modification :