| DOI | Trouver le DOI : https://doi.org/10.1166/jbn.2018.2604 |
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| Auteur | Rechercher : Jin, Wuyang; Rechercher : Lin, Donghai1; Rechercher : Nguyen, Anh H.1; Rechercher : Abdelrasoul, Gaser N.; Rechercher : Chen, Jian1; Rechercher : Mar, Arthur; Rechercher : Qian, Feng; Rechercher : Fang, Qiang; Rechercher : Kovalchuk, Igor; Rechercher : Wang, Yutian; Rechercher : Chen, Jie1 |
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| Affiliation | - Conseil national de recherches du Canada. Nanotechnologie
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| Format | Texte, Article |
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| Sujet | Enhanced Green Fluorescent Protein (EGFP); fluorescein isothiocyanate (FITC); magnetic nanoparticles; primary cortical neurons; transfection |
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| Résumé | The efficient cell transfection method is vital for various biomedical applications, such as the CRISPR-Cas9 technique. Current cell transfection methods, including lipofectamine, calcium phosphate co-precipitation, nucleofection, and viral infection are not equally efficient for various cells and have their disadvantages. In this study, a magnetic nanoparticle (MNP)-based method was introduced for delivering both FITC dye and a functional EGFP gene into easy-to-transfect HEK cells and difficult-to-transfect rat primary cortical neurons. The transfection efficacy could be controlled in both time-dependent and magnetic strength-dependent manner. This cell transfection method could have substantial potential for targeted drug delivery. |
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| Date de publication | 2018-09-01 |
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| Maison d’édition | American Scientific Publishers |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 38373a4d-82df-4b51-ba12-af9bb99ea5f3 |
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| Enregistrement créé | 2019-05-03 |
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| Enregistrement modifié | 2020-03-16 |
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