| Téléchargement | - Voir le manuscrit accepté : Gene expression atlas of embryo development in Arabidopsis (PDF, 1.6 Mio)
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| DOI | Trouver le DOI : https://doi.org/10.1007/s00497-019-00364-x |
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| Auteur | Rechercher : Gao, Peng1; Rechercher : Xiang, Daoquan1; Rechercher : Quilichini, Teagen D.1; Rechercher : Venglat, Prakash1; Rechercher : Pandey, Prashant K.1; Rechercher : Wang, Edwin; Rechercher : Gillmor, C. Stewart; Rechercher : Datla, Raju1 |
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| Affiliation | - Conseil national de recherches du Canada. Développement des cultures et des ressources aquatiques
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| Format | Texte, Article |
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| Sujet | embryogenesis; embryo isolation; RNA-seq; transcriptome; bioinformatics; Arabidopsis |
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| Résumé | Embryogenesis represents a critical phase in the life cycle of flowering plants. Here, we characterize transcriptome landscapes associated with key stages of embryogenesis by combining an optimized method for the isolation of developing Arabidopsis embryos with high-throughput RNA-seq. The resulting RNA-seq datasets identify distinct overlapping patterns of gene expression, as well as temporal shifts in gene activity across embryogenesis. Network analysis revealed stage-specific and multi-stage gene expression clusters and biological functions associated with key stages of embryo development. Methylation-related gene expression was associated with early- and middle-stage embryos, initiation of photosynthesis components with the late embryogenesis stage, and storage/energy-related protein activation with late and mature embryos. These results provide a comprehensive understanding of transcriptome programming in Arabidopsis embryogenesis and identify modules of gene expression corresponding to key stages of embryo development. This dataset and analysis are a unique resource to advance functional genetic analysis of embryo development in plants. |
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| Date de publication | 2019-02-14 |
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| Maison d’édition | Springer |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRC_ACRD-56424 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 38b5e1d4-6070-44b9-ae29-778718beb005 |
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| Enregistrement créé | 2019-03-05 |
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| Enregistrement modifié | 2020-06-03 |
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