| DOI | Trouver le DOI : https://doi.org/10.1016/j.aquaculture.2004.02.001 |
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| Auteur | Rechercher : Perez-Casanova, J. C.1; Rechercher : Murray, H. M.2; Rechercher : Gallant, J. W.3; Rechercher : Ross, N. W.4; Rechercher : Douglas, S. E.3; Rechercher : Johnson, S. C.2 |
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| Affiliation | - Conseil national de recherches du Canada
- Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
- Conseil national de recherches du Canada. Thérapeutique en santé humaine
- Conseil national de recherches du Canada. Programme d'aide à la recherche industrielle
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| Format | Texte, Article |
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| Sujet | bile salt-activated lipase; fish larvae; gene expression; haddock; pancreas |
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| Résumé | In the present work, we report the characterization of the partial cDNA sequence of a bile salt-activated lipase (BAL) cDNA from haddock. The predicted polypeptide encoded by the cDNA sequence contains the bile salt-binding site characteristic of all BALs at amino acid positions 36–45, and the lipid-binding site thus far only reported in fish BALs starting at position 345. Other features of BAL are also present including: the active site serine motif at positions 111–117, the catalytic triad formed by the residues S114, D239 and H358, and an N-glycosylation site at position 107. The relative levels of BAL gene expression were determined in haddock larvae during ontogeny by reverse transcription–polymerase chain reaction (RT–PCR) with the earliest detectable transcript levels identified at hatch. Using in situ hybridization, the BAL transcripts were localized consistently in the pancreas of haddock larvae from mouth opening until 401 degree days (DD). Using biochemical techniques, the specific activity of BAL was found to decline significantly over time. Our results also suggest that haddock larvae are capable of digesting lipids at the time of mouth opening. |
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| Date de publication | 2004-06-01 |
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| Conditions d’utilisation | - Crown Copyright D 2004 Published by Elsevier B.V. All rights reserved.
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NRCC 42432
1329 |
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| Numéro NPARC | 3538113 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 38f8d279-64ae-46c3-991f-db091b1c20f4 |
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| Enregistrement créé | 2009-03-01 |
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| Enregistrement modifié | 2020-04-17 |
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