| DOI | Trouver le DOI : https://doi.org/10.1016/j.yexcr.2009.09.001 |
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| Auteur | Rechercher : Twomey, Erin1; Rechercher : Li, Yan1; Rechercher : Lei, Joy1; Rechercher : Sodja, Caroline1; Rechercher : Ribecco-Lutkiewicz, Maria1; Rechercher : Smith, Brandon1; Rechercher : Fang, Hung1; Rechercher : Bani-Yaghoub, Mahmud1; Rechercher : McKinnell, Iain1; Rechercher : Sikorska, Marianna1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | SIAH2; MYPT1; Proteasomal pathway; Myosin; Neurons; Glia |
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| Résumé | Myosin phosphatase target subunit 1 (MYPT1), together with catalytic subunit of type1 δ isoform (PP1cδ) and a small 20-kDa regulatory unit (M20), form a heterotrimeric holoenzyme, myosin phosphatase (MP), which is responsible for regulating the extent of myosin light chain phosphorylation. Here we report the identification and characterization of a molecular interaction between Seven in absentia homolog 2 (SIAH2) and MYPT1 that resulted in the proteasomal degradation of the latter in mammalian cells, including neurons and glia. The interaction involved the substrate binding domain of SIAH2 (aa 116–324) and a central region of MYPT1 (aa 445–632) containing a degenerate consensus Siah-binding motif RLAYVAP (aa 493–499) evolutionally conserved from fish to humans. These findings suggest a novel mechanism whereby the ability of MP to modulate myosin light chain might be regulated by the degradation of its targeting subunit MYPT1 through the SIAH2-ubiquitin-proteasomal pathway. In this manner, the turnover of MYPT1 would serve to limit the duration and/or magnitude of MP activity required to achieve a desired physiological effect. |
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| Date de publication | 2010-01-01 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 15462306 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 3a2b2a1d-ac16-4386-85e6-59e0023ecb9f |
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| Enregistrement créé | 2010-08-16 |
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| Enregistrement modifié | 2020-04-17 |
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