| DOI | Trouver le DOI : https://doi.org/10.1002/cmmi.292 |
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| Auteur | Rechercher : Jin, A. Y.; Rechercher : Tuor, U. I.1; Rechercher : Rushforth, D.1; Rechercher : Filfil, R.2; Rechercher : Kaur, J.; Rechercher : Ni, F.2; Rechercher : Tomanek, B.1; Rechercher : Barber, P. A. |
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| Affiliation | - Conseil national de recherches du Canada. Institut du biodiagnostic du CNRC
- Conseil national de recherches du Canada. Institut de recherche en biotechnologie du CNRC
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| Format | Texte, Article |
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| Sujet | magnetic resonance imaging; P-selectin; stroke; neuroinflammation; iron oxide; nanoparticle |
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| Résumé | We have developed a magnetic resonance molecular imaging method using a novel iron-oxide contrast agent targeted towards P-selectin – MNP-PBP (magnetic nanoparticle-P-selectin binding peptide) – to image endothelial activation following cerebral ischemia/reperfusion. MNP-PBP consists of ∼1000 PBP ligands (primary sequence: GSIQPRPQIHNDGDFEEIPEEYLQ GGSSLVSVLDLEPLDAAWL) conjugated to a 50 nm diameter aminated dextran iron oxide particle. In vitro P- and E-selectin binding was assessed by competition ELISA. Transient focal cerebral ischemia was induced in male C57/BL 6 mice followed by contrast injection (MNP-PBP; MNP-NH2; Feridex; MNP-PBP-FITC) at 24 h after reperfusion and T2 magnetic resonance imaging at 9.4 T was performed. Infarction and microvasculature accumulation of contrast agent was assessed in coronal brain sections. MNP-PBP attenuated antibody binding to P-selectin by 34.8 ± 1.7 percent. P-selectin was preferentially increased in the infarct hemisphere and MNP-PBP-FITC accumulation in the infarct hemisphere microvasculature was observed. Compared with the nontargeted iron oxide agents MNP-NH2 and Feridex, MNP-PBP showed a significantly greater T2 effect within the infarction. MR imaging of P-selectin expression with a targeted iron oxide nanoparticle contrast agent may reveal early endothelial activation in stroke and other neuroinflammatory states. |
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| Date de publication | 2009-11-25 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro NPARC | 20109990 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 3a9f9443-8260-43ea-ba5d-e62bb4ea6c5f |
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| Enregistrement créé | 2012-06-09 |
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| Enregistrement modifié | 2020-04-16 |
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