| DOI | Trouver le DOI : https://doi.org/10.1080/15321810701603443 |
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| Auteur | Rechercher : Nielsen, K.; Rechercher : Yu, W.; Rechercher : Lin, M.; Rechercher : Davis, S.; Rechercher : Elmgren, C.; Rechercher : MacKenzie, Colin1; Rechercher : Tanha, Jamshid1; Rechercher : Li, S.1; Rechercher : Dubuc, G.1; Rechercher : Brown, E.; Rechercher : Keleta, L.; Rechercher : Pasick, J. |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | Lateral flow technology, Avian influenza virus, Chicken antibody |
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| Résumé | A rapid and effective lateral flow assay (LFA) for detection of avian influenza virus (AIV) was developed. For antigen capture, the assay used monoclonal antibody specific for a conserved nuclear protein (NP) epitope, immobilized on a cellulose acetate matrix, in conjunction with a second NP monoclonal antibody chemically linked to either coloured latex beads or colloidal gold particles contained in a sample pad for detection. Virus sample added to the sample pad flowed into the trapping antibody to form a visible band as well as a second, control band further along the acetate strip. The control band consisted of recombinant protein A/G, also immobilized on the matrix. A second LFA for detection of chicken antibody to AIV was developed where NP antigen was immobilized on the matrix with recombinant protein A/G immobilized as a control band. Latex beads or colloidal gold particles to which monoclonal anti-chicken antibody was attached, were used as the indicator system. |
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| Date de publication | 2007-09-27 |
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| Dans | |
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| Langue | anglais |
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| Publications évaluées par des pairs | Oui |
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| Numéro du CNRC | NIELSEN2007 |
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| Numéro NPARC | 9379036 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 3f19441b-c629-46bd-bccc-0d34eba0f416 |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-05-10 |
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