| Auteur | Rechercher : Tauskela, Joseph S.1; Rechercher : Mealing, Geoff1; Rechercher : Comas, Tanya1; Rechercher : Brunette, Eric1; Rechercher : Monette, Robert1; Rechercher : Small, Daniel L.1; Rechercher : Morley, Paul1 |
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| Affiliation | - Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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| Format | Texte, Article |
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| Sujet | 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid; 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid; acid; anaerobiosis; calcium; Canada; cell survival; cells, cultured; cerebral cortex; cytology; dose-response relationship, drug; drug effects; female; glucose; glutamic acid; membrane potentials; metabolism; N-methylaspartate; neurons; oxygen; pharmacology; physiology; pregnancy; protection; rats; rats, sprague-dawley; receptor; support, Non-U. S. Gov't; toxicity |
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| Résumé | The Cl(-) channel blockers, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) or 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS) dose-dependently protected against oxygen-glucose deprivation in cultured rat cortical neurons. DIDS or SITS attenuated oxygen-glucose deprivation-induced increases in extracellular glutamate concentrations and intracellular Ca(2+). DIDS or SITS provided moderate protection against N-methyl-D-aspartate (NMDA) toxicity and decreased NMDA receptor-mediated increases in intracellular Ca(2+). Whole-cell NMDA receptor currents were attenuated 39+/-2% and 21+/-3% by 1 mM DIDS and SITS, respectively. Other Cl(-) channel blockers as equipotent as DIDS and SITS did not decrease oxygen-glucose deprivation- or NMDA-mediated neuronal Ca(2+) influx or toxicity. Neurotoxicity by exogenous glutamate was not prevented by SITS and was exacerbated by DIDS. Reductions in oxygen-glucose deprivation-induced increases in intracellular Ca(2+) levels underlie neuroprotection by DIDS and SITS. This was a reflection of lower extracellular [glutamate], direct inhibition of Ca(2+) influx through postsynaptic NMDA receptors, and possibly through other protective properties associated with DIDS and SITS |
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| Date de publication | 2003-03-07 |
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| Dans | |
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| Langue | anglais |
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| Numéro du CNRC | TAUSKELA2003A |
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| Numéro NPARC | 9382806 |
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| Exporter la notice | Exporter en format RIS |
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| Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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| Identificateur de l’enregistrement | 43a21616-c017-456d-b5fe-6ae04b5a2e81 |
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| Enregistrement créé | 2009-07-10 |
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| Enregistrement modifié | 2020-04-02 |
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