Téléchargement | - Voir le manuscrit accepté : Cell to aperture interaction in Patch-Clamp Chips visualized by Fluorescence microscopy and Focused-Ion Beam sections (PDF, 1.0 Mio)
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DOI | Trouver le DOI : https://doi.org/10.1002/bit.23127 |
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Auteur | Rechercher : Py, Christophe1; Rechercher : Salim, Danish1; Rechercher : Monette, Robert2; Rechercher : Comas, Tanya2; Rechercher : Fraser, Jeffrey1; Rechercher : Martinez, Dolores1; Rechercher : Martina, Marzia2; Rechercher : Mealing, Geoffrey2 |
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Affiliation | - Conseil national de recherches du Canada. Institut des sciences des microstructures du CNRC
- Conseil national de recherches du Canada. Institut des sciences biologiques du CNRC
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Format | Texte, Article |
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Sujet | ion channels; planar patch-clamp chip; gigaseal; fluorescence microscopy; focused ion beam; scanning electron microscopy |
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Résumé | Patch-clamp is an important method to monitor the electrophysiological activity of cells and the role of pharmacological compounds on specific ion channel roteins. In recent years, planar patch-clamp chips have been developed as a higher throughput approach to the established glass-pipette method. However, proper conditions to optimize the high resistance cell-to-probe seals required to measure the small currents resulting from ion channel activity are still the subject of conjecture. Here, we report on the design of multiple-aperture (sieve) chips to rapidly facilitate assessment of cell-to-aperture interactions in statistically significant numbers. We propose a method to prescreen the quality of seals based on a dye loading protocol through apertures in the chip and subsequent evaluation with fluorescence confocal microscopy. We also show the first scanning electron micrograph of a focused ion beam section of a cell in a patch-clamp chip aperture. |
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Date de publication | 2011-03-09 |
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Dans | |
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Langue | anglais |
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Publications évaluées par des pairs | Oui |
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Numéro NPARC | 18074298 |
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Exporter la notice | Exporter en format RIS |
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Signaler une correction | Signaler une correction (s'ouvre dans un nouvel onglet) |
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Identificateur de l’enregistrement | 460ff7b3-d06c-495d-9314-2f2adc0684b7 |
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Enregistrement créé | 2011-06-23 |
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Enregistrement modifié | 2020-04-21 |
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