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Enzymatic hydrolysis of the esterfied diarrhetic shellfish poisoning toxins and pectenotoxins

Par Conseil national de recherches du Canada

DOITrouver le DOI : https://doi.org/10.1007/s00216-007-1489-3
AuteurRechercher : 1; Rechercher : 1; Rechercher : 1
Affiliation
  1. Conseil national de recherches du Canada. Institut des biosciences marines du CNRC
FormatTexte, Article
Sujetdiarrhetic shellfish poisoning (DSP); okadaic acid; dinophysistoxins; pectenotoxins; esters; liquid chromatography–mass spectrometry; enzymatic hydrolysis
Résumé
Okadaic acid (OA) and dinophysistoxins-1 and -2 (DTX1, DTX2), the toxins responsible for incidents of diarrhetic shellfish poisoning (DSP), can occur as complex mixtures of ester derivatives in both plankton and shellfish. Alkaline hydrolysis is usually employed to release parent OA/DTX toxins, and analyses are conducted before and after hydrolysis to determine the concentrations of nonesterified and esterified toxins. Recent research has shown that other toxins, including pectenotoxins and spirolides, can also exist as esters in shellfish, but these toxins cannot survive alkaline hydrolysis. A promising alternative approach is enzymatic hydrolysis. In this study, two enzymatic methods were developed for the hydrolysis of 7-O-acyl esters, “DTX3,” and the carboxylate esters of OA, “diol-esters.” Porcine pancreatic lipase induced complete conversion of DTX3 to OA and DTXs within one hour for reference solutions. The presence of mussel tissue matrix reduced the rate of hydrolysis, but an optimized lipase concentration resulted in greater than 95% conversion within four hours. OA-diol-ester was hydrolyzed by porcine liver esterase and was completely converted to OA in less than 30 min, even in the presence of mussel tissue matrix. Esters and OA/DTX toxins were all monitored by LC–MS. Further experiments with pectenotoxin esters indicated that enzymatic hydrolysis could also be applied to esters of other toxins. Enzymatic hydrolysis has excellent potential as an alternative to the conventional alkaline hydrolysis procedure used in the preparation of shellfish samples for the analysis of toxins.
Date de publication
Dans
  • Analytical and Bioanalytical Chemistry 389, nº 1 (28 juillet 2007) : 335–342.
Langueanglais
Publications évaluées par des pairsOui
Numéro du CNRCNRCC 42735
1293
Numéro NPARC3538291
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Identificateur de l’enregistrement466688ae-095a-495e-9669-f37b4bea35b0
Enregistrement créé2009-03-01
Enregistrement modifié2020-05-10
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